Product Description
Mouse FSTL3(Follistatin Like Protein 3) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: FLRG; FSRP; Follistatin-Related Protein,Secreted Glycoprotein; Follistatin-related gene protein
Species: Mouse
Assay Type: Sandwich
Sensitivity: 6.4 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: serum, plasma and other biological fluids
Assay Length: 4.5h
Research AreaResearch Area: Reproductive science;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Follistatin Like Protein 3(FSTL3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Follistatin Like Protein 3(FSTL3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Follistatin Like Protein 3(FSTL3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Follistatin Like Protein 3(FSTL3) in the samples is then determined by comparing the OD of the samples to the standard curve.
