Product Description
Mouse FceRI(Receptor I For The Fc Region Of Immunoglobulin E) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: FCER1A; FCE1A; FcER1 High affinity immunoglobulin epsilon receptor subunit alpha
Species: Mouse
Assay Type: Sandwich
Sensitivity: 0.057 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: tissue homogenates, cell lysates and other biological fluids
Assay Length: 4.5h
Research AreaResearch Area: Signal transduction;CD & Adhesion molecule;Infection immunity;Dermatology;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Receptor I For The Fc Region Of Immunoglobulin E(FceRI). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Receptor I For The Fc Region Of Immunoglobulin E(FceRI). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Receptor I For The Fc Region Of Immunoglobulin E(FceRI), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Receptor I For The Fc Region Of Immunoglobulin E(FceRI) in the samples is then determined by comparing the OD of the samples to the standard curve.
