Product Description
Mouse F13B(Coagulation Factor XIII B Polypeptide) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: F13-B; FXIIIB; Fibrin-stabilizing factor B subunit; Protein-glutamine gamma-glutamyltransferase B chain; Transglutaminase B chain
Species: Mouse
Assay Type: Sandwich
Sensitivity: 1.44 ng/mL
Standard: 200 ng/mL
Detection Range: 3.13-200 ng/mL
Sample Type: Plasma .
Assay Length: 4.5h
Research AreaResearch Area: Signal transduction;Metabolic pathway;Hematology;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Coagulation Factor XIII B Polypeptide(F13B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Coagulation Factor XIII B Polypeptide(F13B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Coagulation Factor XIII B Polypeptide(F13B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Coagulation Factor XIII B Polypeptide(F13B) in the samples is then determined by comparing the OD of the samples to the standard curve.
