Product Description
Mouse Estrogen ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: E ;Estrogen
Species: Mouse
Assay Type: Competitive Inhibition
Sensitivity: 4.15 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.625-1000 pg/mL
Sample Type: Serum, plasma, tissue homogenates and other biological fluids.
Assay Length: 2h
Research AreaResearch Area: Reproductive science;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Estrogen protein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Estrogen. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Estrogen in the samples is then determined by comparing the OD of the samples to the standard curve.
