Product Description
Mouse CPA3(Carboxypeptidase A3, Mast Cell) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: MC-CPA; Mast cell carboxypeptidase A
Species: Mouse
Assay Type: Sandwich
Sensitivity: 0.054 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: Serum, plasma and other biological fluids.
Assay Length: 4.5h
Research AreaResearch Area: Enzyme & Kinase;Infection immunity;Rheumatology;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Carboxypeptidase A3, Mast Cell(CPA3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Carboxypeptidase A3, Mast Cell(CPA3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Carboxypeptidase A3, Mast Cell(CPA3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Carboxypeptidase A3, Mast Cell(CPA3) in the samples is then determined by comparing the OD of the samples to the standard curve.
