Product Description
Mouse CH25H(Cholesterol-25-Hydroxylase) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: h25OH; Cholesterol 25-monooxygenase
Species: Mouse
Assay Type: Sandwich
Sensitivity: 0.27 ng/mL
Standard: 50 ng/mL
Detection Range: 0.79-50 ng/mL
Sample Type: Tissue homogenates, cell lysates and other biological fluids
Assay Length: 4.5h
Research AreaResearch Area: Enzyme & Kinase;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cholesterol-25-Hydroxylase(CH25H). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cholesterol-25-Hydroxylase(CH25H). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cholesterol-25-Hydroxylase(CH25H), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cholesterol-25-Hydroxylase(CH25H) in the samples is then determined by comparing the OD of the samples to the standard curve.
