Product Description
Mouse CD34(Cluster Of Differentiation 34) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: Hematopoietic progenitor cell antigen CD34
Species: Mouse
Assay Type: Sandwich
Sensitivity: 0.069 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 4.5h
Research AreaResearch Area: CD & Adhesion molecule;Developmental science;Genetic science;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cluster Of Differentiation 34(CD34). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cluster Of Differentiation 34(CD34). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cluster Of Differentiation 34(CD34), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cluster Of Differentiation 34(CD34) in the samples is then determined by comparing the OD of the samples to the standard curve.
