Product Description
Mouse Bcl9(B-Cell CLL/Lymphoma 9) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: LGS; Protein legless homolog
Species: Mouse
Assay Type: Sandwich
Sensitivity: 27 pg/mL
Standard: 5000 pg/mL
Detection Range: 78.13-5000 pg/mL
Sample Type: Serum, plasma, tissue homogenates and other biological fluids.
Assay Length: 4.5h
Research AreaResearch Area: Immune molecule;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to B-Cell CLL/Lymphoma 9(Bcl9). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to B-Cell CLL/Lymphoma 9(Bcl9). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain B-Cell CLL/Lymphoma 9(Bcl9), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of B-Cell CLL/Lymphoma 9(Bcl9) in the samples is then determined by comparing the OD of the samples to the standard curve.
