Product Description
Mouse APAF1(Apoptotic Peptidase Activating Factor 1) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: CED4; Apoptotic Protease Activating Factor
Species: Mouse
Assay Type: Sandwich
Sensitivity: 3.1 pg/mL
Standard: 500 pg/mL
Detection Range: 7.82-500 pg/mL
Sample Type: Tissue homogenates, cell lysates and other biological fluids.
Assay Length: 4.5h
Research AreaResearch Area: Apoptosis;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Apoptotic Peptidase Activating Factor 1(APAF1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Apoptotic Peptidase Activating Factor 1(APAF1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Apoptotic Peptidase Activating Factor 1(APAF1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Apoptotic Peptidase Activating Factor 1(APAF1) in the samples is then determined by comparing the OD of the samples to the standard curve.
