Product Description
Mouse 20S-PSM(20S-Proteasome) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: CP; 20SPSM; 20S Core Particle
Species: Mouse
Assay Type: Competitive Inhibition
Sensitivity: 152.4 ng/mL
Standard: 30000 ng/mL
Detection Range: 468.75-30000 ng/mL
Sample Type: Serum, plasma and other biological fluids.
Assay Length: 2h
Research AreaResearch Area:
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 20S-Proteasome(20S-PSM) protein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to 20S-Proteasome(20S-PSM). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 20S-Proteasome(20S-PSM) in the samples is then determined by comparing the OD of the samples to the standard curve.
