RDI Divison of researchd Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.
rabbit anti-human Alkaline Phosphatase (human intestine)
cat#RDI-ALKPHOSHabr $375.00/50mg
cat#RDI-ALKPHOSH-HRP $438.00/20mg HRP conjugated
cat#RDI-ALKPHOSH-BT $438.00/10mg Biotin conjugated
-reacts with alkaline phosphatase from human intestine showing a 60kda band against this enzyme under reducing conditions of SDS-PAGE. Reactivity with ther sources unknown.
DATA SHEET: (ALK Phos calf intestine)
Rabbit anti-Alkaline Phosphatase Catalog#: RDI-ALKPHOSabr $312.00/50mg
Sheep anti-Alkaline Phosphatase RDI-ALKPHOSabS $312.00/50mg
Goat anti-Alkaline Phosphatase RDI-ALKPHOSabG $315.00/50mg
Also available HRP conjugated: cat# add -HRP $344.00/20mg
-custom FITC and PE conjugates available-please inquire
Size: 50 mg (lyophilized)
Antibody Concentration: see batch sheet
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride,pH 7.2
Stabilizer: None
Preservative: 0.01% (w/v) Sodium Azide
Antigen: calf intestine alkaline phosphatase
Specificity: no cross is observed against the bacterial enzyme. Cross reactivity will occur with alkaline phosphatases from other mammals and tissues.
Applications: suitable for immunoblotting (western or dot blot),ELISA, immunoprecipitation, conjugation and most immunological methods requiring high titer and specificity.
Recommended Dilutions: Not determined. Optimal titers for applications should be determined by the researcher.
Storage Conditions: Store vial at 4'C prior to restoration. Re-store with 5.0 ml of deionized water (or equivalent). Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4'C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20'C or below. Avoid cycles of freezing and thawing. Expiration date is one (1)year from date of reconstitution.
For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.
Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.
MOUSE MONOCLONAL ANTIBODY TO: HUMAN ALKALINE PHOSPHATASE
PRODUCT CODE RDI-CBL119 $375.00/1mg
CLONE 327
ISOTYPE IgG1
IMMUNIZING ANTIGEN Calf intestinal alkaline phosphatase
FUSION PARTNER NSO myeloma cell line
SOURCE Tissue culture supernatant
PURIFICATION METHOD Protein A affinity chromatography
SPECIFICITY The antibody reacts with alkaline phosphatase from a number of species and specific for the intestinal enzyme form.
APPLICATIONS *Used in an APAAP complex for immunohistochemistry
*ELISA development as a detection antibody
LOT SPECIFIC DATA RDI-CBL119 CONT'D
PRESENTATION The monoclonal is presented at a concentration of 1mg in phosphate buffered saline containing 10mM sodium azide. We recommend that each laboratory determine an optimum working titre for use in its particular application.
STORAGE For use within 1 month of purchase store at +4oC, for long term storage aliquot antibody into small volumes and store at -20oC.
Bulk quotes on request
For Research Use Only. Not supplied for use in diagnostic or therapeutic procedures.
DATA SHEET: Rabbit anti-Alkaline Phosphatase (E.Coli)
Catalog#: RDI-ALKPHOSECabr $312.00/vial
Also available HRP conjugated: cat# add -HRP $344.00/20mg
Size: 50 mg (lyophilized)
Antibody Concentration: 50mg lyophilized
Buffer: 0.02 M Potassium Phosphate, 0.15 M
Sodium Chloride,pH 7.2
Stabilizer: None
Preservative: 0.01% (w/v) Sodium Azide
Antigen: E. Coli alkaline phosphatase
Specificity: reacts with alkaline phosphatase from E. Coli (cross reactivity may occur with other bacterial sources) No cross observed with mammalian enzyme.
Applications: suitable for immunoblotting (western or dot blot),ELISA, immunoprecipitation, conjugation and most immunological methods requiring high titer and specificity.
Recommended Dilutions: Not determined. Optimal titers for applications should be determined by the researcher.
Storage Conditions: Store vial at 4'C prior to restoration Re-store with 5.0 ml of deionized water (or equivalent). Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4'C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquotcontents and freeze at -20'C or below. Avoid cycles of freezing and thawing. Expiration date is one (1)year from date of reconstitution.
For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.
Note: None of these antibodies has been tested with other isoforms of alkaline phosphatase. Generally, if you pretreat your sample with levamisole, this will greatly reduce most non intestinal alkaline phosphatase activity.