Product Description
MAX (phospho-S11) polyclonal antibody is available at gentaur for next week delivery
Background: It is now well established that the nature and relative abundance of individual subunits of different classes of transcription factors can positively or negatively regulate levels of gene expression. Myc proteins homodimerize and bind DNA poorly, if at all, at physiological levels. Max is a nuclear localized bHLH-Zip protein initially identified by screening a B cell expression library with the bHLH-Zip region of c-Myc. Max homodimers and the Myc-Max heterodimers bind the sequence CACGTG; however the binding of the hetero-dimeric complex is stronger than the Max homodimer. The Max gene products have been identified as Max and Max 9 proteins that differ by a 9 amino acid insertion N-terminal to the basic region. In contrast to Myc, which is highly regulated during progression through the cell cycle, Max is highly stable and is much more abundant than Myc. Two members of the bHLH-Zip protein family, designated Mad and Mxi1, homodimerize poorly but form heterodimeric complexes with Max that have opposing functions to Myc-Max heterodimers with respect to regulation of gene expression.
Applications: WB IF
Purification&Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: P-MAX(S11) polyclonal antibody detects endogenous levels of MAX protein only when phosphorylated at Ser11.
W4BiowMW: ~ 21 kDa
Reactivity: Human
Note: For research use only, not for use in diagnostic procedure.
Immunogen:
Synthetic phosphopeptide derived from human MAX around the phosphorylation site of Serine 11.Alternative Name:
Protein max; Class D basic helix-loop-helix protein 4; bHLHd4; Myc-associated factor X; BHLHD4Western blot analysis: Western blot analysis of extracts of Jurkat cells, using Phospho-MAX-S11 antibody.
Immunohistochemistry:
Immunofluorescence analysis: Immunofluorescence analysis of U2OS cells, using MAX (phospho-S11) polyclonal antibody
Host: Rabbit
Swiss-Prot: P61244
"
