Product Description
MAPKAPK-2 (phospho-S272) polyclonal antibody is available at gentaur for next week delivery
Background: In response to cytokines, stress, and chemotactic factors, MAP kinase-activated protein kinase 2 (MAPKAPK-2) is rapidly phosphorylated and activated. It has been shown that MAPKAPK-2 is a direct target of p38 MAPK. Multiple residues of MAPKAPK-2 are phosphorylated in vivo in response to stress. However, only four residues (Thr25, Thr222, Ser272, and Thr334) are phosphorylated by p38 MAPK in an in vitro kinase assay. Phosphorylation at Thr222, Ser272, and Thr334 appears to be essential for the activity of MAPKAPK-2. Thr25 is phosphorylated by p42 MAPK in vitro, but is not required for the activation of MAPKAPK-2.
Applications: WB
Purification&Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: MAPKAPK-2 (phospho-S272) polyclonal antibody detects endogenous levels of MAPKAPK-2 protein only when phosphorylated at Ser272.
W4BiowMW: ~ 47, 49 kDa
Reactivity: Human,Rat,Mouse
Note: For research use only, not for use in diagnostic procedure.
Immunogen:
Synthetic phosphopeptide derived from human MAPKAPK-2 around the phosphorylation site of Serine 272Alternative Name:
MAP kinase-activated protein kinase 2; MAPK-activated protein kinase 2; MAPKAP kinase 2; MAPKAP-K2; MAPKAPK-2; MK-2; MK2; MAPKAPK2Western blot analysis: Western blot (WB) analysis of MAPKAPK-2 (phospho-S272) polyclonal antibody at 1:500 dilution Lane1:Hela whole cell lysate(40ug) Lane2:The Spleen tissue lysate of Mouse(40ug) Lane3:The Spleen tissue lysate of Rat(40ug)
Immunohistochemistry:
Immunofluorescence analysis:
Host: Rabbit
Swiss-Prot: P49137
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