mAb Mouse Anti-his tag

Catalog number:

Akr-003

Quantity and concentration:

100 µg of protein a purified antibody at 1 mg/ml in 10 mm pbs, ph 7.2, containing 0.05% nan3

Shelf life:

1 year from date of receipt under proper storage conditions; aliquot to avoid multiple freeze thaw cycles

Host species:

Mouse

Isotype:

Igg2b

Clone:

His.h8

Immunogen:

6xhis synthetic peptide conjugated to klh

Specificity:

 recognizes the epitope of n-terminal, c-terminal, or internal 6xhis-tags

Sensitivity:

1-2 ng of his-tagged fusion proteins (immunoblot/ecl)

Application:

Immunoblot (1:1000 to 1:3000; incubate 1 hr at room temp), immunostaining (1:500 to 1:2000) elisa, dot blot or immunoprecipitation (user to determine working dilution)

Storage:

-20ºc