Product Description
M-RIP polyclonal antibody is available at gentaur for next week delivery
Background: Myosin phosphatase-rho interacting protein (M-RIP), also known as p116RIP, RIP3, and MPRIP, localizes to actin-myosin filaments regulating cytoskeletal dynamics. M-RIP contains amino-terminal pleckstrin homology domains, carboxyl-terminal coiled-coil domains, and was originally identified to associate with the myosin phosphatase complex. M-RIP binds to MBS/MYRT, the myosin binding subunit of myosin phosphatase, as well as RhoA. Phosphorylation of MYRT by Rho-associated kinase (ROCK) inhibits myosin phosphatase activity, resulting in increased levels of phosphorylation on myosin light chain, and enhanced contractility. M-RIP may function as a scaffolding protein for the complex between the myosin phosphatase complex, Rho/ROCK, and actin. Silencing of M-RIP results in disassembly of the complex, increased phosphorylation of myosin light chain, and changes to cytoskeletal dynamics.
Applications: WB IP IHC
Purification&Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: M-RIP polyclonal antibody detects endogenous levels of M-RIP protein.
W4BiowMW: ~ 130-160kDa
Reactivity: Human,Mouse,Rat
Note: For research use only, not for use in diagnostic procedure.
Immunogen:
Recombinant protein of human M-RIP.Alternative Name:
Myosin phosphatase Rho-interacting protein; M-RIP;Rho-interacting protein 3;RIP3;p116Rip;MPRIPWestern blot analysis: Western blot (WB) analysis of M-RIP polyclonal antibody at 1:5000 dilution Lane1:PC12 whole cell lysate(40ug) Lane2:CT-26 whole cell lysate(40ug) Lane3:H1792 whole cell lysate(40ug) Lane4:Myla2059 whole cell lysate(40ug) Lane5:PC3 whole cell lysate(40ug) Lane6:SGC7901 whole cell lysate(40ug)
Immunohistochemistry: Immunoprecipitation - M-RIP Polyclonal Antibody
Immunofluorescence analysis: Immunohistochemistry of paraffin-embedded human colon carcinoma using M-RIP antibody at dilution of 1:50.
Host: Rabbit
Swiss-Prot: Q6WCQ1
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