Product Description
IRS-1 (Ser794) polyclonal antibody is available at gentaur for next week delivery
Background: Insulin receptor substrate 1 (IRS-1) is one of the major substrates of the insulin receptor kinase. IRS-1 contains multiple tyrosine phosphorylation motifs that serve as docking sites for SH2-domain containing proteins that mediate the metabolic and growth-promoting functions of insulin. IRS-1 also contains over 30 potential serine/threonine phosphorylation sites. Ser307 of IRS-1 is phosphorylated by JNK and IKK while Ser789 is phosphorylated by SIK-2, a member of the AMPK family. The PKC and mTOR pathways mediate phosphorylation of IRS-1 at Ser612 and Ser636/639, respectively. Phosphorylation of IRS-1 at Ser1101 is mediated by PKCθ and results in an inhibition of insulin signaling in the cell, suggesting a potential mechanism for insulin resistance in some models of obesity.
Applications: WB
Purification&Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: IRS-1 (Ser794) polyclonal antibody detects endogenous levels of IRS-1 protein.
W4BiowMW: ~ 180 kDa
Reactivity: Human
Note: For research use only, not for use in diagnostic procedure.
Immunogen:
Synthetic peptide, corresponding to amino acids 764-824 of Human IRS-1.Alternative Name:
Insulin receptor substrate 1; IRS-1; IRS1Western blot analysis: Western blot (WB) analysis of IRS-1 (Ser794) polyclonal antibody at 1:2000 dilution Lane1:HepG2 whole cell lysate(40ug) Lane2:A2780 whole cell lysate(40ug) Lane3:A375 whole cell lysate(40ug)
Immunohistochemistry:
Immunofluorescence analysis:
Host: Rabbit
Swiss-Prot: P35568
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