IRS-1 (phospho-Y612) polyclonal antibody

Product Description

IRS-1 (phospho-Y612) polyclonal antibody is available at gentaur for next week delivery

Background: IRS-1, a major substrate of the insulin receptor, is phosphorylated in response to stimulation of cells by insulin, insulin-like growth factor 1 (IGF-1) and interleukin 4 (IL-4). IRS-1 is phosphorylated on serine, threonine and tyrosine residues in a variety of tissues. An insulin-sensitive serine/threonine kinase casein kinase II mediates a portion of the insulin-stimulated serine/threonine phosphorylation of overexpressed IRS-1 in vivo. Thr 502 is identified as the major casein kinase II-catalyzed phosphorylation site in rat IRS-1, and Ser 99 is an additional phosphorylation site catalyzed by casein kinase II. Thus, casein kinase II-catalyzed phosphorylation of IRS-1 may be a component of the intracellular insulin signaling cascade. IRS-1 contains three putative binding sites for 14-3-3 (Ser 270, Ser 374 and Ser 641) and the motif around Ser 270 is located in the phosphortyrosine binding domain of IRS-1, which is responsible for the interaction with the insulin receptor.

Applications:  WB IHC

Purification&Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability:  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity:  p-IRS-1 (Y612) polyclonal antibody detects endogenous levels of IRS-1 protein only when phosphorylated at Ser612.

W4BiowMW:  ~ 180 kDa

Reactivity: Human,Mouse,Rat

Note:  For research use only, not for use in diagnostic procedure.

Immunogen:

Synthetic phosphopeptide derived from human IRS-1 around the phosphorylation site of Tyrosine 612.

Alternative Name:

Insulin receptor substrate 1; IRS-1; IRS1

Western blot analysis:  Western blot (WB) analysis of p-IRS-1 (Y612) pAb at 1:500 dilution Lane1:MCF-7 whole cell lysate(40ug) Lane2:HEK293T whole cell lysate(40ug) Lane3:A375 whole cell lysate(40ug) Lane4:A2780 whole cell lysate(40ug) Lane5:Hela whole cell lysate(40ug) Lane6:C6 whole cell lysate(40ug) Lane7:NIH-3T3 whole cell lysate(40ug)

Immunohistochemistry: Immunohistochemistry (IHC) analyzes of p-IRS-1 (Y612) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Immunofluorescence analysis:

Host:  Rabbit

Swiss-Prot: P35568

 

 

 

 

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