iNOS polyclonal antibody

Product Description

iNOS polyclonal antibody is available at gentaur for next week delivery

Background: Nitric oxide (NO) has a broad range of biological activities and has been implicated in signaling pathways in phylogenetically diverse species. Nitric oxide synthases (NOSs), the enzymes responsible for synthesis of NO, contain an N-terminal oxygenase domain and a C-terminal reductase domain. NOS activity requires homodimerization as well as three cosubstrates (L-arginine, NADPH and O2) and five cofactors or prosthetic groups (FAD, FMN, calmodulin, tetrahydrobiopterin and heme). Several distinct NOS isoforms have been described and been shown to represent the products of three distinct genes. These include two constitutive Ca2+/CaM-dependent forms of NOS, including NOS1 (also designated ncNOS) whose activity was first identified in neurons, and NOS3 (also designated ecNOS), first identified in endothelial cells. The inducible form of NOS, NOS2 (also designated iNOS), is Ca2+-independent and is expressed in a broad range of cell types.

Applications:  WB IHC IF

Purification&Purity: Peptide affinity purified

Storage&Stability:  Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Specificity:  iNOS polyclonal antibody detects endogenous levels of iNOS protein.

W4BiowMW:  ~ 131 kDa

Reactivity: Human, Mouse

Note:  For research use only, not for use in diagnostic procedure.

Immunogen:

Peptide

Alternative Name:

HEP-NOS, Inducible NOS, iNOS, NOS2, NOS2A, HEP-NOS, Hepatocyte NOS, HEPNOS, inducible, Inducible nitric oxide synthase, Inducible NO synthase, Inducible NOS, iNOS, MAC NOS, Macrophage NOS, Nitric oxide synthase 2 inducible, Nitric oxide synthase 2 inducible macrophage, nitric oxide synthase 2A (inducible, hepatocytes), Nitric oxide synthase, Nitric oxide synthase inducible, nitric oxide synthase, macrophage, NOS 2, NOS, Nos II, NOS type II, nos2, NOS2, NOS2A, NOS2A, Inducible, Hepatocyte, Peptidyl-cysteine S-nitrosylase NOS2,

Western blot analysis:  Western blot analysis of extracts of RAW264.7 cells, using NOS2 antibody at 1:1000 dilution.Raw264.7 cells were treated by LPS (1 μg/ml) at 37℃ for over night.

Immunohistochemistry: Immunohistochemistry of paraffin-embedded mouse kidney using NOS2 antibody at dilution of 1:100 (40x lens).

Immunofluorescence analysis: Immunofluorescence analysis of U2OS cells using NOS2 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

Host:  Rabbit

Swiss-Prot: P35228

 

 

 

 

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