Immunoprecipitation

Anti-MOUSE  CD35  Antibodies

 Rat anti mouse CD35 (CR1,CD21b) , monoclonal

  Catalog#: RDI-MCD35-8C12    $312.00/vial     $250.00/vial 3+   Bulk on request

-also available Biotin labeled cat#RDI-MCD35-8CBT $562.00/0.5mg

Package Size: 0.1mg in 0.2ml (0.5mg/ml) 10mM phosphate   buffer pH7.2 with 150mM NaCl & 0.09 %NaN3

Clone: 8C12

Ig Isotype: rat IgG2ak

Immunogen: purified mouse CR1

Reactivity: Reacts with an epitope present on the 190-kda complement receptor protein, originally designated CR1 (CD35), but not on the 145-150Kda CR2 (CD21) molecule. Unlike the human system, in which these proteins are products of independent genes, both of these mouse receptors are membrane proteins resulting from the alternating splicing of mRNA transcribed from the Cr2 gene. Therefore an alternative nomenclature has been proposed, designating the proteins Cr2-190 (CD21b) and Cr2-145 (CD21a) respectively. The epitope recognized by 8C12 Mab is only present on CD35/CD21b. Morever, it has also been proposed that Crry is the true mouse genetic homologue of human CR1(CD35). In the mouse, CD35 is expressed on the majority of peripheral B cells,on the majority of resident peritoneal macrophages, on peripheral blood granulocytes after treatement with N-formyl-Met-Leu-Phe, and on follicular dendritic cells, but not on thymocytes, T cells, erythrocytes or platelets. In addition, it has not been detected, at protein or mRNA level, in the   macrophage cell line J774, bone marrow or platelets. Mab 8C12 has been reported to inhibit rosette formation by C3B-bearing sheep erythrocytes, to block the complmement-dependent trapping of immune complexes by follicular dendritic cells, and to down-regulate mouse CD35 expression upon invivo application,inhibiting only some primary antibody responses to immunization. B lymphocytes of Cr2(null) display impaired humoral immune responses invivo. The 8C12 Mab recognizes an epitope on mouse CD35 distinct from the epitope recognized by anti-mouse CD21/Cd25 clone 7G6 and it does not block binding by 7G6 to CD35.

USE: -flow cytometry(<1ug/1M cells)

         -histochemistry (acetone fixed frozen sections)

         -immunoprecipitation

          -western blot

           -in vitro and in vivo blocking of CR1 mediated functions

Storage: Store 4 Deg C.

Precautions:For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. Sodium azide may form explosive compounds in presence of heavy metals or under acidic conditions. Flush drains with copious amounts of water to prevent buildup of explosive compounds.