Product Description
IL6 polyclonal antibody is available at gentaur for next week delivery
Background: Acute phase response is induced by interleukin-6 (IL-6) produced by T cells, macrophages, fibroblasts, endothelial and other cells . IL-6 induces proliferation or differentiation in many cell types including B cells, thymocytes and T cells. IL-6, in concert with TGF-β, is important for developing Th17 responses. IL-6 binds to IL-6Rα and through this association induces gp130 homodimerization . gp130 homodimerization triggers the Jak/Stat cascade and the SHP-2/Erk MAP kinase cascade . IL-6 also forms a complex with an IL-6Rα splice variant that is nonmembrane-associated . The IL-6/soluble IL-6Rα complex can then activate the gp130 signaling pathway in cells that express gp130 but not IL-6Rα . Research studies have shown that IL-6, through increasing expression of proangiogenic VEGF, may also contribute to metastatic breast cancer.
Applications: WB
Purification&Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 96% (by SDS-PAGE).
Storage&Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: IL6 polyclonal antibody detects endogenous levels of IL6 protein.
W4BiowMW: ~ 28 kDa
Reactivity: Human,Rat,Mouse
Note: For research use only, not for use in diagnostic procedure.
Immunogen:
Synthetic peptide, corresponding to Human IL6.Alternative Name:
Interleukin-6; IL-6; B-cell stimulatory factor 2; BSF-2; CTL differentiation factor; CDF; Hybridoma growth factor; Interferon beta-2; IFN-beta-2; IL6; IFNB2Western blot analysis: Western blot (WB) analysis of IL6 polyclonal antibody at 1:500 dilution Lane1:Hela whole cell lysate(40ug) Lane2:U-87MG whole cell lysate(40ug) Lane3:HuT78 whole cell lysate(40ug) Lane4:A549 whole cell lysate(40ug) Lane5:MCF-7 whole cell lysate(40ug)
Immunohistochemistry:
Immunofluorescence analysis:
Host: Rabbit
Swiss-Prot: P05231
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