IKKα/β (D173) polyclonal antibody

Product Description

IKKα/β (D173) polyclonal antibody is available at gentaur for next week delivery

Background: The NF-κB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IκB proteins. Most agents that activate NF-κB do so through a common pathway based on phosphorylation-induced, proteasome-mediated degradation of IκB. The key regulatory step in this pathway involves activation of a high molecular weight IκB kinase (IKK) complex whose catalysis is generally carried out by three tightly associated IKK subunits. IKKα and IKKβ serve as the catalytic subunits of the kinase and IKKγ serves as the regulatory subunit. Activation of IKK depends upon phosphorylation at Ser177 and Ser181 in the activation loop of IKKβ (Ser176 and Ser180 in IKKα), which causes conformational changes, resulting in kinase activation.

Applications:  WB IHC

Purification&Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability:  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity:  IKKα/β (D173) polyclonal antibody detects endogenous levels of IKKα/β protein.

W4BiowMW:  ~ 85, 97 kDa

Reactivity: Human,Mouse,Rat

Note:  For research use only, not for use in diagnostic procedure.

Immunogen:

Synthetic peptide, corresponding to amino acids 151-200 of Human IKKα

Alternative Name:

I kappa-B kinase alpha; IkBKA; IKK-alpha; IKK-A; IkappaB kinase; EC 2.7.11.10; I-kappa-B kinase 1; IKK1; Conserved helix-loop-helix ubiquitous kinase; Nuclear factor NF-kappa-B inhibitor kinase alpha; NFKBIKA; CHUK; IKKA; TCF16

Western blot analysis:  Western blot (WB) analysis of IKKα/β (D173) pAb at 1:500 dilution Lane1:MEF whole cell lysate(40ug) Lane2:H9C2 whole cell lysate(40ug) Lane3:Panc1 whole cell lysate(40ug) Lane4:PC3 whole cell lysate(40ug) Lane5:Jurkat whole cell lysate(40ug)

Immunohistochemistry: Immunohistochemistry (IHC) analyzes of IKKα/β (D173) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.showing Membrane and Cytoplasmic staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Immunofluorescence analysis: Immunohistochemistry (IHC) analyzes of IKKα/β (D173) pAb in paraffin-embedded human tonsil carcinoma tissue at 1:50.showing Membrane and Cytoplasmic staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Host:  Rabbit

Swiss-Prot: O15111/O14920

 

 

 

 

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