Product Description
ID3 polyclonal antibody is available at gentaur for next week delivery
Background: Members of the Id family of basic helix-loop-helix (bHLH) proteins include Id1 (1–3), Id2 (4), Id3 and Id4 (5). They are ubiquitously expressed and dimerize with members of the class A and B HLH proteins. Due to the absence of the basic region, the resulting heterodimers cannot bind DNA. The Id-type proteins thus appear to negatively regulate DNA binding of bHLH proteins. Since Id1 inhibits DNA binding of E12 and Myo D, it apparently functions to inhibit muscle-specific gene expression. Under conditions that facilitate muscle cell differentiation, the Id protein levels fall, allowing E12 and/or E47 to form heterodimers with Myo D and myogenin, which in turn activate myogenic differentiation. It has been shown that expression of each of the Id proteins is strongly dependent on growth factor activation and that reduction of Id mRNA levels by antisense oligonucleotides leads to a delayed reentry of arrested cells into the cell cycle following growth factor stimulation.
Applications: WB IHC IF
Purification&Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: ID3 polyclonal antibody detects endogenous levels of ID3 protein.
W4BiowMW: Refer to figures
Reactivity: Human,Mouse,Rat
Note: For research use only, not for use in diagnostic procedure.
Immunogen:
Recombinant full length Human ID3.Alternative Name:
1R21; bHLHb25; Class B basic helix-loop-helix protein 25; DNA binding protein inhibitor ID 3; DNA-binding protein inhibitor ID-3; HEIR 1; HEIR1; Helix loop helix protein HEIR 1; Helix loop helix protein HEIR1; Helix-loop-helix protein HEIR-1; HLH642; ID 3; ID like protein inhibitor HLH 1R21; ID like protein inhibitor HLH 462; ID-like protein inhibitor HLH 1R21; ID3; ID3_HUMAN; Inhibitor of DNA binding 3; Inhibitor of DNA binding 3 dominant negative helix loop helix protein;Western blot analysis:
Immunohistochemistry: Immunohistochemistry of paraffin-embedded rat brain using ID3 antibody at dilution of 1:200 (400x lens).
Immunofluorescence analysis: Immunohistochemistry of paraffin-embedded mouse kidney using ID3 antibody at dilution of 1:200 (400x lens).
Host: Rabbit
Swiss-Prot: Q02535
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