Product Description
IARS2 polyclonal antibody is available at gentaur for next week delivery
Background: The fidelity of protein synthesis requires efficient discrimination of amino acid substrates by aminoacyl-tRNA synthetases. Accurate discrimination of the structurally similar amino acids valine and isoleucine by isoleucyl-tRNA synthetase (IleRS) results, in part, from a hydrolytic editing reaction, which prevents misactivated valine from being stably joined to tRNAIle. IleRS joins Ile to tRNA(Ile) at its synthetic active site and hydrolyzes incorrectly acylated amino acids at its editing active site. A member of the aminoacyl-tRNA synthetase family, human IleRS has been identified as a target of antibodies in the autoimmune disease polymyositis.
Applications: WB IHC IF
Purification&Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: IARS2 polyclonal antibody detects endogenous levels of IARS2 protein.
W4BiowMW: ~ 114 kDa
Reactivity: Human,Mouse,Rat
Note: For research use only, not for use in diagnostic procedure.
Immunogen:
Synthetic peptide, corresponding to Human IARS2.Alternative Name:
Isoleucine--tRNA ligase, mitochondrial; Isoleucyl-tRNA synthetase; IleRS; IleRSWestern blot analysis: Western blot (WB) analysis of IARS2 polyclonal antibody at 1:500 dilution LaneA:HEK293T whole cell lysate LaneB:Hela whole cell lysate LaneC:H446 whole cell lysate LaneD:The Kidney tissue lysate of Mouse LaneE:The Testis tissue lysate of Mouse LaneF:The Kidney tissue lysate of Rat LaneG:The Testis tissue lysate of Rat
Immunohistochemistry:
Immunofluorescence analysis:
Host: Rabbit
Swiss-Prot: Q9NSE4
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