Product Description
Human UBE3A(Ubiquitin Protein Ligase E3A) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: ANCR; AS; E6-AP; EPVE6AP; HPVE6A; Papilloma Virus E6-Associated,Angelman Syndrome; Oncogenic protein-associated protein E6-AP; Renal carcinoma antigen NY-REN-54
Species: Human
Assay Type: Sandwich
Sensitivity: 0.119 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: tissue homogenates, cell lysates and other biological fluids
Assay Length: 4.5h
Research AreaResearch Area: Enzyme & Kinase;Developmental science;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ubiquitin Protein Ligase E3A(UBE3A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ubiquitin Protein Ligase E3A(UBE3A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ubiquitin Protein Ligase E3A(UBE3A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ubiquitin Protein Ligase E3A(UBE3A) in the samples is then determined by comparing the OD of the samples to the standard curve.
