Product Description
Human TNNI1(Troponin I Type 1, Slow Skeletal) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: TNN1; Troponin I, slow-twitch isoform
Species: Human
Assay Type: Sandwich
Sensitivity: 17.59 pg/mL
Standard: 3000 pg/mL
Detection Range: 46.88-3000 pg/mL
Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Assay Length: 4.5h
Research AreaResearch Area: Cardiovascular biology;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Troponin I Type 1, Slow Skeletal(TNNI1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Troponin I Type 1, Slow Skeletal(TNNI1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Troponin I Type 1, Slow Skeletal(TNNI1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Troponin I Type 1, Slow Skeletal(TNNI1) in the samples is then determined by comparing the OD of the samples to the standard curve.
