Product Description
Human TAT(Thrombin/Antithrombin Complex) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names:
Species: Human
Assay Type: Sandwich
Sensitivity: 2.87 pg/mL
Standard: 400 pg/mL
Detection Range: 6.25-400 pg/mL
Sample Type: Plasma
Assay Length: 4.5h
Research AreaResearch Area: Signal transduction;Enzyme & Kinase;Metabolic pathway;Hematology;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Thrombin/Antithrombin Complex(TAT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Thrombin/Antithrombin Complex(TAT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Thrombin/Antithrombin Complex(TAT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Thrombin/Antithrombin Complex(TAT) in the samples is then determined by comparing the OD of the samples to the standard curve.
