Product Description
Human TAC1(Tachykinin, Precursor 1) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: NK2; NKNA; NKA; TAC2; •PPT; Protachykinin-1; Substance P; Neurokinin A; Neuromedin L; Substance K; Neuropeptide K; Neuropeptide gamma; C-terminal-flanking peptide
Species: Human
Assay Type: Competitive Inhibition
Sensitivity: 52.8 pg/mL
Standard: 10000 pg/mL
Detection Range: 156.25-10000 pg/mL
Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 2h
Research AreaResearch Area: Neuro science;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Tachykinin, Precursor 1(TAC1) protein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tachykinin, Precursor 1(TAC1). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Tachykinin, Precursor 1(TAC1) in the samples is then determined by comparing the OD of the samples to the standard curve.
