Product Description
Human SPR(Sepiapterin Reductase) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: SDR38C1; 7,8-Dihydrobiopterin:NADP+ Oxidoreductase; Short Chain Dehydrogenase/Reductase Family 38C,Member 1
Species: Human
Assay Type: Sandwich
Sensitivity: 0.108 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: Tissue homogenates and other biological fluids.
Assay Length: 4.5h
Research AreaResearch Area: Enzyme & Kinase;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sepiapterin Reductase(SPR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Sepiapterin Reductase(SPR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sepiapterin Reductase(SPR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sepiapterin Reductase(SPR) in the samples is then determined by comparing the OD of the samples to the standard curve.
