Product Description
Human SEMG1(Semenogelin I) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: SEMG; SGI; CT103; Semen Coagulating Protein; Cancer/Testis Antigen 103; Seminal basic protein
Species: Human
Assay Type: Sandwich
Sensitivity: 6.2 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: serum, plasma, seminal plasma and other biological fluids
Assay Length: 4.5h
Research AreaResearch Area: Metabolic pathway;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Semenogelin I(SEMG1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Semenogelin I(SEMG1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Semenogelin I(SEMG1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Semenogelin I(SEMG1) in the samples is then determined by comparing the OD of the samples to the standard curve.
