Product Description
Human RXRa(Retinoid X Receptor Alpha) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: NR2B1; RXR-A; NR2-B1; Nuclear Receptor Subfamily 2,Group B,Member 1; Retinoic acid receptor RXR-alpha
Species: Human
Assay Type: Sandwich
Sensitivity: 0.117 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: Tissue homogenates, cell lysates and other biological fluids.
Assay Length: 4.5h
Research AreaResearch Area: Signal transduction;Metabolic pathway;Endocrinology;Hormone metabolism;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Retinoid X Receptor Alpha(RXRa). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Retinoid X Receptor Alpha(RXRa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Retinoid X Receptor Alpha(RXRa), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Retinoid X Receptor Alpha(RXRa) in the samples is then determined by comparing the OD of the samples to the standard curve.
