Product Description
Human REG1b(Regenerating Islet Derived Protein 1 Beta) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: PSPS2; REGH; REGI-BETA; REGL; Pancreatic Stone Protein; Pancreatic Thread Protein; Lithostathine 1 Beta; Secretory Pancreatic Stone Protein 2
Species: Human
Assay Type: Sandwich
Sensitivity: 0.65 ng/mL
Standard: 100 ng/mL
Detection Range: 1.57-100 ng/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 4.5h
Research AreaResearch Area: Infection immunity;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Regenerating Islet Derived Protein 1 Beta(REG1b). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Regenerating Islet Derived Protein 1 Beta(REG1b). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Regenerating Islet Derived Protein 1 Beta(REG1b), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Regenerating Islet Derived Protein 1 Beta(REG1b) in the samples is then determined by comparing the OD of the samples to the standard curve.
