Product Description
Human PXR(Pregnane X Receptor) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: NR1I2; SAR; PAR1; PAR2; PRR; BXR; ONR1; PAR; PARq; SXR; Nuclear Receptor Subfamily 1,Group I,Member 2; Steroid and xenobiotic receptor; Pregnane X receptor
Species: Human
Assay Type: Sandwich
Sensitivity: 0.126 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: Tissue homogenates, cell lysates and other biological fluids
Assay Length: 4.5h
Research AreaResearch Area: Signal transduction;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pregnane X Receptor(PXR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Pregnane X Receptor(PXR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pregnane X Receptor(PXR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pregnane X Receptor(PXR) in the samples is then determined by comparing the OD of the samples to the standard curve.
