Product Description
Human ProIAPP(Proislet Amyloid Polypeptide) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: Pro-IAPP; Pro-Amylin; Pro-Islet Amyloid Polypeptide
Species: Human
Assay Type: Competitive Inhibition
Sensitivity: 8.94 pg/mL
Standard: 2000 pg/mL
Detection Range: 31.25-2000 pg/mL
Sample Type: Serum, plasma and other biological fluids
Assay Length: 2h
Research AreaResearch Area: Apoptosis;Infection immunity;Hepatology;Gastroenterology;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Proislet Amyloid Polypeptide(ProIAPP) protein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Proislet Amyloid Polypeptide(ProIAPP). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Proislet Amyloid Polypeptide(ProIAPP) in the samples is then determined by comparing the OD of the samples to the standard curve.
