Product Description
Human PIP(Prolactin Induced Protein) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: GCDFP15; GPIP4; BRST2; SABP; gp17; Gross cystic disease fluid protein 15; Prolactin-induced protein; Secretory actin-binding protein
Species: Human
Assay Type: Sandwich
Sensitivity: 0.255 ng/mL
Standard: 40 ng/mL
Detection Range: 0.63-40 ng/mL
Sample Type: Serum, plasma, tissue homogenates and other biological fluids.
Assay Length: 4.5h
Research AreaResearch Area: Immune molecule;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Prolactin Induced Protein(PIP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Prolactin Induced Protein(PIP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Prolactin Induced Protein(PIP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Prolactin Induced Protein(PIP) in the samples is then determined by comparing the OD of the samples to the standard curve.
