Product Description
Human PIIINP(Procollagen III N-Terminal Propeptide) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: P3NP; N-Propeptide Of Type III Procollagen; Procollagen III Amino Terminal Propeptide
Species: Human
Assay Type: Competitive Inhibition
Sensitivity: 2.13 ng/mL
Standard: 400 ng/mL
Detection Range: 6.25-400 ng/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 2h
Research AreaResearch Area: Metabolic pathway;Hepatology;Bone metabolism;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Procollagen III N-Terminal Propeptide(PIIINP) protein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Procollagen III N-Terminal Propeptide(PIIINP). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Procollagen III N-Terminal Propeptide(PIIINP) in the samples is then determined by comparing the OD of the samples to the standard curve.
