Product Description
Human PDE1A(Phosphodiesterase 1A, Calmodulin Dependent) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: hCam-1; 61 kDa Cam-PDE; Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1A
Species: Human
Assay Type: Sandwich
Sensitivity: 0.126 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: Tissue homogenates and other biological fluids.
Assay Length: 4.5h
Research AreaResearch Area: Enzyme & Kinase;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phosphodiesterase 1A, Calmodulin Dependent(PDE1A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Phosphodiesterase 1A, Calmodulin Dependent(PDE1A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phosphodiesterase 1A, Calmodulin Dependent(PDE1A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Phosphodiesterase 1A, Calmodulin Dependent(PDE1A) in the samples is then determined by comparing the OD of the samples to the standard curve.
