Product Description
Human PARN(Poly A Specific Ribonuclease) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: DAN; Deadenylation Nuclease; Deadenylating nuclease; Polyadenylate-specific ribonuclease
Species: Human
Assay Type: Sandwich
Sensitivity: 0.27 ng/mL
Standard: 50 ng/mL
Detection Range: 0.79-50 ng/mL
Sample Type: Tissue homogenates and other biological fluids.
Assay Length: 4.5h
Research AreaResearch Area: Enzyme & Kinase;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Poly A Specific Ribonuclease(PARN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Poly A Specific Ribonuclease(PARN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Poly A Specific Ribonuclease(PARN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Poly A Specific Ribonuclease(PARN) in the samples is then determined by comparing the OD of the samples to the standard curve.
