Human p-cresol ELISA Kit

Product Description

Human p-cresol ELISA Kit is availabe at Gentaur for next week delivery.

Alternative Names:  4-Hydroxytoluene; 4-Methylphenol; p-Cresol 98+ %; PARA-CRESOL; 4-cresol; para cresol

Species:  Human

Assay Type:  Sandwich

Sensitivity:  1.21 pg/mL

Standard:  200 pg/mL

Detection Range:  3.13-200 pg/mL

Sample Type:  serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Length:  4.5h

Research AreaResearch Area:  Neuro science;

Test Principle:  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to p-cresol. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to p-cresol. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain p-cresol, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of p-cresol in the samples is then determined by comparing the OD of the samples to the standard curve.