Product Description
Human MUC17(Mucin 17) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: MUC3; Mucin Cell Surface Associated; Small intestinal mucin-3
Species: Human
Assay Type: Sandwich
Sensitivity: 6 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: Serum, plasma, tissue homogenates and other biological fluids.
Assay Length: 4.5h
Research AreaResearch Area: Tumor immunity;Infection immunity;Pulmonology;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mucin 17(MUC17). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mucin 17(MUC17). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mucin 17(MUC17), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mucin 17(MUC17) in the samples is then determined by comparing the OD of the samples to the standard curve.
