Product Description
Human MS4A1(Membrane Spanning 4 Domains Subfamily A, Member 1) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: CD20; MS4-A1; B1; Bp35; LEU16; MS4-A2; S7; B-lymphocyte antigen CD20; B-lymphocyte surface antigen B1
Species: Human
Assay Type: Sandwich
Sensitivity: 0.49 ng/mL
Standard: 80 ng/mL
Detection Range: 1.25-80 ng/mL
Sample Type: serum, plasma, tissue homogenates and other biological fluids
Assay Length: 4.5h
Research AreaResearch Area: CD & Adhesion molecule;Tumor immunity;Infection immunity;Immune molecule;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Membrane Spanning 4 Domains Subfamily A, Member 1(MS4A1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Membrane Spanning 4 Domains Subfamily A, Member 1(MS4A1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Membrane Spanning 4 Domains Subfamily A, Member 1(MS4A1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Membrane Spanning 4 Domains Subfamily A, Member 1(MS4A1) in the samples is then determined by comparing the OD of the samples to the standard curve.