Product Description
Human MHCG(Major Histocompatibility Complex Class I G) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: HLA-G; MHC-G; HLAG; Leukocyte Antigen G; b2 microglobulin; HLA class I histocompatibility antigen, alpha chain G
Species: Human
Assay Type: Sandwich
Sensitivity: 0.17 ng/mL
Standard: 24 ng/mL
Detection Range: 0.38-24 ng/mL
Sample Type: serum, plasma, tissue homogenates and other biological fluids
Assay Length: 4.5h
Research AreaResearch Area: Apoptosis;Tumor immunity;Infection immunity;Immune molecule;Hematology;Reproductive science;Immunodeficiency;Autoimmunity;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Major Histocompatibility Complex Class I G(MHCG). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Major Histocompatibility Complex Class I G(MHCG). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Major Histocompatibility Complex Class I G(MHCG), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Major Histocompatibility Complex Class I G(MHCG) in the samples is then determined by comparing the OD of the samples to the standard curve.
