Product Description
Human MEP1b(Meprin A Beta) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: PPH Beta; Endopeptidase-2; Meprin B; PABA Peptide Hydrolase; N-benzoyl-L-tyrosyl-P-amino-Benzoic Acid Hydrolase Subunit Beta
Species: Human
Assay Type: Sandwich
Sensitivity: 0.117 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: Tissue homogenates, cell lysates and other biological fluids.
Assay Length: 4.5h
Research AreaResearch Area: Metabolic pathway;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Meprin A Beta(MEP1b). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Meprin A Beta(MEP1b). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Meprin A Beta(MEP1b), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Meprin A Beta(MEP1b) in the samples is then determined by comparing the OD of the samples to the standard curve.
