Human ME2(Malic Enzyme 2, NADP+ Dependent, Mitochondrial) ELISA Kit

Product Description

Human ME2(Malic Enzyme 2, NADP+ Dependent, Mitochondrial) ELISA Kit is availabe at Gentaur for next week delivery.

Alternative Names: 

Species:  Human

Assay Type:  Sandwich

Sensitivity:  0.054 ng/mL

Standard:  10 ng/mL

Detection Range:  0.16-10 ng/mL

Sample Type:  Tissue homogenates and other biological fluids.

Assay Length:  4.5h

Research AreaResearch Area:  Enzyme & Kinase;

Test Principle:  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Malic Enzyme 2, NADP+ Dependent, Mitochondrial(ME2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Malic Enzyme 2, NADP+ Dependent, Mitochondrial(ME2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Malic Enzyme 2, NADP+ Dependent, Mitochondrial(ME2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Malic Enzyme 2, NADP+ Dependent, Mitochondrial(ME2) in the samples is then determined by comparing the OD of the samples to the standard curve.