Product Description
Human MCL1(Myeloid Cell Leukemia Sequence 1, Bcl2 Related) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: EAT; MCL1L; MCL1S; BCL2L3; TM; Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1; Bcl-2-like protein 3; Bcl-2-related protein EAT/mcl1; mcl1/EAT
Species: Human
Assay Type: Sandwich
Sensitivity: 0.127 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: Tissue homogenates, cell lysates and other biological fluids
Assay Length: 4.5h
Research AreaResearch Area: Tumor immunity;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Myeloid Cell Leukemia Sequence 1, Bcl2 Related(MCL1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Myeloid Cell Leukemia Sequence 1, Bcl2 Related(MCL1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Myeloid Cell Leukemia Sequence 1, Bcl2 Related(MCL1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Myeloid Cell Leukemia Sequence 1, Bcl2 Related(MCL1) in the samples is then determined by comparing the OD of the samples to the standard curve.
