Product Description
Human MASP1(Mannose Associated Serine Protease 1) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: CRARF; PRSS5; Mannan-Binding Lectin Serine Peptidase 1; Ra-reactive factor serine protease p100; Complement-activating component of Ra-reactive factor; Serine protease 5
Species: Human
Assay Type: Sandwich
Sensitivity: 42.57 pg/mL
Standard: 6000 pg/mL
Detection Range: 93.75-6000 pg/mL
Sample Type: serum, plasma and other biological fluids
Assay Length: 4.5h
Research AreaResearch Area: Enzyme & Kinase;Metabolic pathway;Apoptosis;Infection immunity;Hematology;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mannose Associated Serine Protease 1(MASP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mannose Associated Serine Protease 1(MASP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mannose Associated Serine Protease 1(MASP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mannose Associated Serine Protease 1(MASP1) in the samples is then determined by comparing the OD of the samples to the standard curve.
