Product Description
Human MAPRE1(Microtubule Associated Protein RP/EB Family, Member 1) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: EB1; Adenomatous Polyposis Coli-Binding protein; APC-binding protein EB1; End-binding protein 1
Species: Human
Assay Type: Sandwich
Sensitivity: 0.058 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: Tissue homogenates, cell lysates and other biological fluids
Assay Length: 4.5h
Research AreaResearch Area: Metabolic pathway;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Microtubule Associated Protein RP/EB Family, Member 1(MAPRE1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Microtubule Associated Protein RP/EB Family, Member 1(MAPRE1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Microtubule Associated Protein RP/EB Family, Member 1(MAPRE1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Microtubule Associated Protein RP/EB Family, Member 1(MAPRE1) in the samples is then determined by comparing the OD of the samples to the standard curve.
