Product Description
Human LRRFIP1(Leucine Rich Repeat In FLII Interacting Protein 1) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: GCF2; FLAP-1; FLIIAP1; GCF-2; HUFI-1; TRIP; GC-Binding Factor 2; TAR RNA-interacting protein
Species: Human
Assay Type: Sandwich
Sensitivity: 0.127 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: Tissue homogenates and other biological fluids.
Assay Length: 4.5h
Research AreaResearch Area: Immune molecule;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Leucine Rich Repeat In FLII Interacting Protein 1(LRRFIP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Leucine Rich Repeat In FLII Interacting Protein 1(LRRFIP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Leucine Rich Repeat In FLII Interacting Protein 1(LRRFIP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Leucine Rich Repeat In FLII Interacting Protein 1(LRRFIP1) in the samples is then determined by comparing the OD of the samples to the standard curve.
