Product Description
Human LIN28A(Lin-28 Homolog A) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: ZCCHC1; CSDD1; LIN28; Zinc finger CCHC domain-containing protein 1
Species: Human
Assay Type: Sandwich
Sensitivity: 21.9 pg/mL
Standard: 4000 pg/mL
Detection Range: 62.5-4000 pg/mL
Sample Type: tissue homogenates, cell lysates and other biological fluids
Assay Length: 4.5h
Research AreaResearch Area: Signal transduction;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lin-28 Homolog A(LIN28A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lin-28 Homolog A(LIN28A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lin-28 Homolog A(LIN28A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Lin-28 Homolog A(LIN28A) in the samples is then determined by comparing the OD of the samples to the standard curve.
