Product Description
Human LFA3(Lymphocyte Function Associated Antigen 3) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: CD58; Ag3; Surface glycoprotein LFA-3
Species: Human
Assay Type: Sandwich
Sensitivity: 5.8 pg/mL
Standard: 800 pg/mL
Detection Range: 12.5-800 pg/mL
Sample Type: Serum, plasma, cell lysatesand other biological fluids.
Assay Length: 4.5h
Research AreaResearch Area: CD & Adhesion molecule;Tumor immunity;Infection immunity;Immune molecule;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lymphocyte Function Associated Antigen 3(LFA3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lymphocyte Function Associated Antigen 3(LFA3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lymphocyte Function Associated Antigen 3(LFA3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Lymphocyte Function Associated Antigen 3(LFA3) in the samples is then determined by comparing the OD of the samples to the standard curve.
