Product Description
Human LAT(Linker For Activation Of T-Cell) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: LAT1; Pp36; Linker Of Activated T Cells; Linker for activation of T-cells family member 1; 36 kDa phospho-tyrosine adapter protein
Species: Human
Assay Type: Sandwich
Sensitivity: 0.102 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: Tissue homogenates, cell lysates and other biological fluids.
Assay Length: 4.5h
Research AreaResearch Area: Immune molecule;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Linker For Activation Of T-Cell(LAT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Linker For Activation Of T-Cell(LAT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Linker For Activation Of T-Cell(LAT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Linker For Activation Of T-Cell(LAT) in the samples is then determined by comparing the OD of the samples to the standard curve.
